Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference |
Identifiers | Additional information |
|---|---|---|---|---|
| Strain, strain background (Mus musculus) | C57BL/6J | Charles River | ||
| Strain, strain background (M. musculus) | Amhr2-Cre Knock-in | Jamin et al., 2002 | DOI: 10.1038/ng1003 | |
| Strain, strain background (M. musculus) | Gnrh1 < GFP> | Spergel et al., 1999 | DOI:10.1523/JNEUROSCI.19-06-02037.1999 | |
| Recombinant DNA reagent | AMH-His | GeneCust | Seq ref: NM_000479.3 | |
| Recombinant DNA reagent | AMHR2-His | GeneCust | Seq Ref: NM_000479.3 | |
| Recombinant DNA reagent | AMH-p.Thr99Ser-His | This Paper | ||
| Recombinant DNA reagent | AMH-p.Pro151Ser-His | This Paper | ||
| Recombinant DNA reagent | AMH-p.Asp238Glu-His | This Paper | ||
| Recombinant DNA reagent | AMHR2-p.Gly445_Leu453del-His | This Paper | ||
| Cell line | GN11 | Radovick et al., 1991 | Lab Stock |
https://doi.org/10.1073/pnas.88.8.3402
GN11 cells were isolated from a male mouse |
| Cell line | GT1-7 | Mellon et al., 1990 | Lab Stock; RRID:CVCL_0281 |
https://doi.org/10.1016/0896-6273(90)90028-E
GT1-7 cells were isolated from a mouse, unknown sex |
| Cell line | COS-7 | Lab Stock; RRID:CVCL_0224 | COS-7 cells were isolated from a monkey | |
| Transfected construct | Amhr2 SMARTpool siRNA | Dharmacon | #M-053605-00-0005 | |
| Transfected construct | Acvr1 SMARTpool siRNA | Dharmacon | #M-042047-01-0005 | |
| Transfected construct | Bmpr1a SMARTpool siRNA | Dharmacon | # M-040598-01-0005 | |
| Transfected construct | Bmpr1b SMARTpool siRNA | Dharmacon | # M-051071-00-0005 | |
| Transfected construct | Non-targeting siRNA control pool | Dharmacon | # D-001206-13-05 | |
| Antibody | Phospho-ERK1/2 (Thr202/Tyr204) (rabbit) | Cell Signaling | #9101L; RRID:AB_331646 | 1:1000 |
| Antibody | ERK1/2 (Thr202/Tyr204) (rabbit) | Cell Signaling | #9102L; RRID:AB_330744 | 1:1000 |
| Antibody | AMH (mouse) | Abcam | #Ab24542 ; RRID:AB_2801539 |
1:500 |
| Antibody | AMH (rabbit) | Abcam | #Ab103233; RRID:AB_10711946 | 1:500 |
| Antibody | AMHR2 (rabbit) | CASLO | Custom made #56G | 1:2000 |
| Antibody | GnRH (guinea pig) | Dr. Erik Hrabovszky, Institute of Experimental Medicine of the Hungarian Academy of Sciences, Budapest, Hungary | Lab Stock | 1:3000; https://doi.org/10.3389/fendo.2011.00080 |
| Antibody | Peripherin (Contactin1) (rabbit) | Millipore | #AB1530; RRID:AB_90725 | 1:1000 |
| Antibody | β-III tubulin (TUJ-1) (mouse) | Sigma Aldrich | #T8660; RRID:AB_477590 | 1:800 |
| Antibody | AMHR2 Neutralizing Antibody | R&D systems | #AF1618 ; RRID: AB_2226485 |
1:200 |
| Antibody | TAG-1 (goat) | R&D systems | AF2215 | |
| Antibody | Actin (mouse) | Sigma Aldrich | #A5316; RRID:AB_476743 |
1:1000 |
| Antibody | Donkey anti-rabbit IgG AlexaFluor 488 (H + L) | Molecular Probes | #A-21026; RRID:AB_141708 | 1:500 |
| Antibody | Donkey anti-rabbit IgG AlexaFluor 555 (H + L) | Molecular Probes | #A-31572; RRID:AB_162543 | 1:500 |
| Antibody | Donkey anti-mouse IgG AlexaFluor 488 (H + L) | Molecular Probes | #A-21202; RRID:AB_141607 | 1:500 |
| Antibody | Donkey anti-mouse IgG AlexaFluor 555 (H + L) | Molecular Probes | #A-31570; RRID:AB_2536180 | 1:500 |
| Antibody | Donkey anti-goat IgG AlexaFluor 488 (H + L) | Molecular Probes | #A-11055; RRID:AB_142672 | 1:500 |
| Antibody | Donkey anti-goat IgG AlexaFluor 555 (H + L) | Molecular Probes | #A-21432; RRID:AB_141788 | 1:500 |
| Antibody | Donkey anti-goat IgG AlexaFluor 647 (H + L) | Molecular Probes | #A-21447; RRID:AB_141844 | 1:500 |
| Antibody | Donkey anti-guinea pig IgG AlexaFluor 488 (H + L) | Jackson ImmunoResearch | #706-545-148; RRID:AB_2340472 | 1:500 |
| Antibody | Horse anti-mouse IgG peroxidase labelled | Vector | #PI-2000; RRID:AB_2336177 | 1:5000 |
| Sequence-based reagent | Amh Taqman gene expression assay | Thermofisher Scientific | Mm00431795_g1 | |
| Sequence-based reagent | GnRH Taqman gene expression assay | Thermofisher Scientific | Mm01315605 | |
| Sequence-based reagent | Amhr2 Taqman gene expression assay | Thermofisher Scientific | Mm00513847_m1 | |
| Sequence-based reagent | Acvr1 Taqman gene expression assay | Thermofisher Scientific | Mm01331069_m1 | |
| Sequence-based reagent | Bmpr1a Taqman gene expression assay | Thermofisher Scientific | Mm00477650_m1 | |
| Sequence-based reagent | Bmpr1b Taqman gene expression assay | Thermofisher Scientific | Mm03023971_m1 | |
| Sequence-based reagent | Rn18s Taqman gene expression assay | Thermofisher Scientific | Hs99999901-s1 | |
| Sequence-based reagent | Actb Taqman gene expression assay | Thermofisher Scientific | Mm00607939 | |
| Peptide, recombinant protein | Recombinant Human AMH-C Fragment (goat) | R&D systems | #1737 MS; RRID:AB_2273957 | |
| Commercial assay or kit | Papain Dissociation System | Worthington | #LK003150 | |
| Commercial assay or kit | Lipofectamine 2000 | ThermoFisher Scientific | #11668019 | |
| Commercial assay or kit | AMH Access Dxi chemiluminescent immunoassay | Beckman Coulter | #B13127 | |
| Commercial assay or kit | GnRH EIA kit | Phoenix Pharmaceuticals Inc | #EK-040-02CE | |
| Commercial assay or kit | Annexin V Apoptosis Detection Kit | Thermofisher Scientific | #88-8007-74 | |
| Commercial assay or kit | SureSelect All Exon capture V2 | Agilent Technologies | #5190–9493 | |
| Commercial assay or kit | Gentra Puregene Blood Kit | Qiagen | #158389 | |
| Chemical compound, drug | Flurogold Tracer | Sigma Aldrich | #39286 | 1:1500 |
| Chemical compound, drug | MAPK Kinase inhibitor | Calbiochem | #U0126 | 10 μM |
| Software, algorithm | FACSDiva | BD Biosciences | 8.1 | http://www.bdbiosciences.com/sg/instruments/software/downloads/ |
| Software, algorithm | SDS | Applied Biosystems | 2.4.1 | https://www.thermofisher.com/fr/fr/home/technical-resources/software-downloads/applied-biosystems-7900ht-fast-real-timespcr-system.html |
| Software, algorithm | Data Assist | Applied Biosystems | 3.0.1 | https://www.thermofisher.com/fr/fr/home/technical-resources/software-downloads/dataassist-software.html |
| Software, algorithm | ImageJ | NIH | 3.0.1 | https://imagej.net/Welcome |
| Software, algorithm | IMARIS | Bitplane | 9.1 | https://imaris.oxinst.com/ |
| Software, algorithm | Photoshop | Adobe | 4.0 | https://www.adobe.com/la/products/photoshop.html |
| Software, algorithm | Illustrator | Adobe | 4.0 | https://www.adobe.com/products/illustrator.html |
| Software, algorithm | Prism 6 | Graphpad Software | 6.0 | https://www.graphpad.com/scientific-software/prism/ |
| Software, algorithm | Inspector Pro | La Vision Biotec | 4.0 | |
| Software, algorithm | Burrows-Wheeler Alignment Algorithm | http://bio-bwa.sourceforge.net/ | ||
| Software, algorithm | SnpEff | Switch Laboratoty | 4.0 | http://snpeff.sourceforge.net/ |
| Software, algorithm | dbNSFP | Liu et al., 2011 | 2.9 | http://varianttools.sourceforge.net/Annotation/dbNSFP |
| Software, algorithm | Modeller | Webb and Sali, 2016 | 9.2 | https://salilab.org/modeller/ |