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Optical control of ligand binding via distinct photoswitch attachment strategies. Photoswitchable tethered ligands may be presented to target ligand binding domains through previously established strategies of directly binding to the target protein (A), traditionally via cysteine-maleimide linkage, through binding to introduced self-labeling protein tags (B), such as SNAP, CLIP, or Halo-tag, or potentially through immunochemistry with labeled antibodies (C).
