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. Author manuscript; available in PMC: 2020 Aug 1.
Published in final edited form as: Antiviral Res. 2019 May 31;168:134–145. doi: 10.1016/j.antiviral.2019.05.015

Figure 3. ONC201 inhibits HIV-1 p24, gag RNA, DNA, and integrated LTR DNA in macrophages.

Figure 3.

Human MDM were plated in 24-well plates in triplicate and then infected with HIV-1ADA for 24 hours before incubation with ONC201 or ONC201 isomer at 30 μM for 5 days. A, B) Cell lysates were collected and subjected to SDS-PAGE and immunoblotting for HIV-1 p24. Actin was used as the loading control. Densitometric quantifications of p24 in MDM were presented as a ratio to actin and normalized as fold changes to the vehicle control DMSO group. C) Supernatants were collected and subjected to ELISA for HIV-1 p24. Values represent means ± SEM of three biological replicates. D-F) RNA (D) and DNA (E, F) were isolated from the samples. HIV-1 gag was detected through quantitative real time RT-PCR and real time PCR, respectively. Relative HIV-1 gag and LTR DNA levels were determined and standardized with 18S rRNA or GAPDH internal control. Values represent means ± SEM of three biological replicates. ANOVA analysis: * denotes p < 0.05 compared to the vehicle control DMSO group.