Skip to main content
. Author manuscript; available in PMC: 2020 Aug 1.
Published in final edited form as: Antiviral Res. 2019 May 31;168:134–145. doi: 10.1016/j.antiviral.2019.05.015

Figure 7. Antiviral effect of ONC201 in HIV-1 infected macrophages is dependent on TRAIL expression.

Figure 7.

HIV-1-infected MDM were incubated with ONC201 or ONC201 isomer at 30 μM, along with 100ng/ml, 50ng/ml soluble TRAIL receptor, or 100ng/ml soluble TNF receptor as a control for 5 days. A) HIV-1 infection levels were determined by RTase activity assay. B) Cell lysates were subjected to SDS-PAGE and immunoblotting for HIV-1 p24. Actin was used as the loading control. Densitometric quantifications of p24 in MDM were presented as a ratio to actin and normalized as fold changes to the control group. ANOVA analysis: *** denotes p < 0.001, compared to the control group with isomer treatment; # denotes p < 0.05, ## denotes p < 0.01, compared to the control group with ONC201 treatment.