. 2019 Jul 11;20(Suppl 7):536. doi: 10.1186/s12864-019-5847-2

Table 2.

Occurrence of chimeric BC–ROI combinations under different PCR conditions for high-diversity MPRA libraries

Type of PCR	Elongation time, s^a	MPRA library		Total read count		Reads with chimeric BC–ROI combinations, %
Type of PCR	Elongation time, s^a	name	size^b	R#1	R#2	R#1	R#2
emulsion	10	library-71	22,621	526,056	497,217	0.580	0.555
	30	library-71	51,650	594,511	514,941	0.342	0.310
	30	library-83	38,156	371,889	456,380	0.261	0.304
conventional	30	library-71	85,249	776,878	784,138	0.336	0.337
conventional	30	library-83	52,654	788,801	809,402	0.295	0.310

^aIndicated elongation time was used in both rounds of PCR

^bThe number of genuine BCs found in both replicates

R#1 and R#2 stand for replicate 1 and replicate 2, respectively