Table 3.
Oligonucleotide primers used in the study
| Primer name | Sequence 5′ → 3′ | Notes |
|---|---|---|
| Libr-A1-for | TCGTCGGCAGCGTCAGATGTGTATAAGAGACAG TTCGGAGT GACACTCGAGGATCGAG | Illumina sequencing primer sites “seq1” and “seq2” are underlined by single and double lines, respectively. |
| Libr-A2-for | TCGTCGGCAGCGTCAGATGTGTATAAGAGACAG ACTCATTT GACACTCGAGGATCGAG | |
| Libr-A3-for | TCGTCGGCAGCGTCAGATGTGTATAAGAGACAG GGGATCCG GACACTCGAGGATCGAG | |
| The 8-bp sample index (“i”) sequences are in bold. | ||
| Libr-A5-for | TCGTCGGCAGCGTCAGATGTGTATAAGAGACAG CAAGATAA GACACTCGAGGATCGAG | |
| “Target specific forward” and “target specific reverse” sequences immediately flanking BC and ROI are in italic. | ||
| Libr-A6-for | TCGTCGGCAGCGTCAGATGTGTATAAGAGACAG GGACAACG GACACTCGAGGATCGAG | |
| Libr-A7-for | TCGTCGGCAGCGTCAGATGTGTATAAGAGACAG AGCGAGCT GACACTCGAGGATCGAG | |
| Libr-A8-for | TCGTCGGCAGCGTCAGATGTGTATAAGAGACAG CTGCACGT GACACTCGAGGATCGAG | |
| Libr-A9-for | TCGTCGGCAGCGTCAGATGTGTATAAGAGACAG GCACTAGT GACACTCGAGGATCGAG | |
| Libr-rev | GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG CCCTAGAAAGATAATCATATTGT | |
| Libr-P5-for | AATGATACGGCGACCACCGAGATCTACACTCGTCGGCAGCGTC | Illumina “P5” and “P7” adapters are underlined by single and double lines, respectively. |
| Libr-P7-rev | CAAGCAGAAGACGGCATACGAGATGTCTCGTGGGCTCGG | |
| BC1 | ACTAGGCAAGGCACCAG | |
| BC2 | CGAGGGATATAGAGCGAGTTAA | |
| ROI1 | TACGTTAAAGATAATCATGCATAAGTCG | |
| ROI2 | TACGTTAAAGATAATCATGCTATATGGC | |
| Illumina-qPCR-1 | AATGATACGGCGACCACCGA | KAPA Library Quantification Kit (Roche). |
| Illumina-qPCR-2 | CAAGCAGAAGACGGCATACGA |