Fig. 1.

a Liver’s histiocytes showing intracytoplasmic crystalloid inclusions (black arrow) and extracellular crystals (white arrows) within periportal space (H–E staining × 10 000). Interstitial diffuse infiltration of histiocytes with intracytoplasmic crystalloid inclusions (arrows) in splenic parenchyma (b), BM (c) and lymph node (d) (b–d H–E staining × 4000). e Higher magnification of histiocytes in splenic parenchyma (H–E staining × 6000). Immunohistochemistry shows negative reactivity of the histiocytes (arrows) for kappa (f) and lambda (g) light chains (f, g × 4000). h Positive reactivity for CD68 in histiocytes of BM (× 4000). i Crystals (arrow) show negative birefringence under polarized light (× 2000). j Ultrastructural examination of the spleen shows intracytoplasmic electron dense hexagonal-like crystals, without periodic organization of the substructure (× 25,000)