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. 2019 Jul 10;9:10001. doi: 10.1038/s41598-019-46452-w

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Local pericardial cooling improves myocardial function four weeks post MI. Transthoracic echocardiography was performed prior to, two and four weeks post MI in female farm pigs divided in a warm (n = 3, white) and cold (n = 4, black) group. (A) EF measured by Simpson method prior to MI, two and four weeks post MI. (B) Linear correlation between EF (Simpson method) four weeks post MI and LV temperature. (C) Four-chamber view of the LV used for the Simpson method. (D,E) FS and EF obtained in M-Mode, with the Teichholz formula, four weeks post MI. (F) M-Mode image in the parasternal long axis through the LV in both groups four weeks post MI. (G–I) Evaluation of diastolic function by measuring E’(cm/sec), E/E’ ratio and calculated LAP (mmHg) respectively, using the combination of PW and TVI four weeks post MI. (J) Illustration of PW and TVI respectively. The data represents the mean ± SEM with *p < 0.05, ***p < 0.001 comparing cold vs. warm.