Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Jul 10;9:10001. doi: 10.1038/s41598-019-46452-w

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Mitochondrial mass and function are increased under hypothermia. LV border zone tissue from female farm pigs (n = 3 per group) was harvested one week post MI. WB analyses were performed on the whole lysate. All proteins were normalized to ponceau. (A) WB analysis with protein quantification (B–F) of NDUFB8, SDHB, COXI, ATP5A and TOM70 respectively. Mitochondrial respirometry analyses were performed on fresh isolated mitochondria of LV tissue (n = 7) 4 weeks post MI. (G) Respiratory spare capacity and respirometry trace (H) of the normal and border zone in the warm and cold group. (I) Representative EM images of mitochondria (x19000) demonstrating a disruption of density and structure of mitochondria in the warm condition after MI compared to the cold. The data represents the mean ± SEM with *p < 0.05, **p < 0.01, comparing cold (black) vs. warm (white). We normalized each protein to the entire corresponding lane on the ponceau-stained membrane, representing the total protein amount. Due to space limitation, the blots are cropped from various gels, please see the supplemental data for full-length gels/blots.