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. 2019 Jul 10;9:10007. doi: 10.1038/s41598-019-46316-3

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of MenSCs on NK cell cytotoxicity. (A) IL-2-activated NK cells were cultured with IFN-γ/IL-1β-activated MenSCs for 5 days. Cytotoxicity of NK cells on K562 cells at different ratios was then assessed by cAM fluorimetric assay. IFN-γ-pretreated BMSCs served as positive control. (B,C) Effect of MenSCs co-culturing on the expression of cytotoxic markers was evaluated by flow cytometry. Results are represented as mean ± SEM of 6 MenSCs and 4 BMSCs from different donors. P values less than 0.05, 0.01, and 0.001 are shown with *, ** and, ***, respectively.