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. 2019 Jul 10;9:10007. doi: 10.1038/s41598-019-46316-3

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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NK cell-mediated MenSCs lysis. (A) NK cells were co-cultured with allogeneic cAM-labeled MenSCs from 6 donors in the presence of IL-2, and the level of cytotoxicity was measured at different time points by fluorimetry. (B) Viable cells were monitored under a fluorescent microscope at different time points. (C) IL-2-activated NK cells from 3 different female donors were cultured with 3 allogeneic (MHC Mismatch) or syngeneic (MHC Match) MenSCs. The level of cytotoxicity was then measured as above. Images were photographed by an Olympus microscope (BX-51) equipped with a CCD camera (DP71) camera.