Figure 2.

Expression of CAR, CD19, and genes associated with immune functions in BM samples before and after CAR-T infusion

A. Heatmap of immune microenvironment and immunostimulator genes. The blue to red coloring in the legend indicates the gene expression level (Z score scaled) from low to high. Hierarchical clustering analysis was carried out by calculating the Euclidean distances. The categories of the microenvironment and immunostimulator genes determined by gene functions are indicated below the heatmap. B. The distribution of immune cell populations within the BM of patients among patients on D0 and D14. C. Heatmap of marker genes of B cells (including pro-B and pre-pro-B cells) and key genes related to the cytotoxic function of CD8⁺ T cell. D. Pearson's correlation of membrane protein genes for CD19 and CAR expression (P < 0.05, |correlation| ≥0.5). The red dot indicates the gene that was found to be involved in leukemia. E. The expression levels of the CAR and CD19 genes in the BM of patients on D0 and D14. All the data shown in this figure were based on RNA-seq analyses. TNF, tumor necrosis factor; TNFR, TNF receptor; TLR, toll like receptor; IMP, tissue inhibitor of metalloproteinase; MMP, matrix metallopeptidase; IL, interleukin; ILR, IL receptor; IFNR, interferon alpha and beta receptor; GF, growth factor; GFR, GF receptor; NK, natural killer.