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Minocycline suppresses LPS-induced retinal microglia activation through downregulation of TLR4 signaling. A: Real-time PCR analysis of Toll-like receptor (TLR) 2 and TLR4 mRNA expression in BV-2 microglial cells exposed to 100 ng/ml of lipopolysaccharide (LPS) with or without minocycline (50 μg/ml) for 6 h (n=5 per group). *p<0.05 compared with the LPS treated with PBS group. B: Western blotting of TLR2 and TLR4 protein expression (n=4 per group).
