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. 2019 Jul 10;39(7):BSR20182196. doi: 10.1042/BSR20182196

Figure 4. Up-regulated miR-613 or down-regulated FN1 contributes to suppressed cell proliferation, invasion, and migration, and promoted cell apoptosis of CNE1 cells.

Figure 4

(A) Cell proliferation of CNE1 cells after treatments with the mimic or inhibitor of miR-613 and FN1 as well as the LY294002 treatment detected by EdU (200×); (B) cell apoptosis of CNE1 cells after treatments with the mimic or inhibitor of miR-613 and FN1 as well as the LY294002 treatment examined by flow cytometry; (C) the protein bands of Bcl-2, Bax, Cleaved-caspase3 in CNE1 cells examined by Western blot analysis; (D) cell migration of CNE1 cells after treatments with the mimic or inhibitor of miR-613 and FN1 as well as the LY294002 treatment detected by Transwell assay (200×); (E) cell invasion of CNE1 cells after treatments with the mimic or inhibitor of miR-613 and FN1 as well as the LY294002 treatment detected by Transwell assay (200×); (F) protein expression of MMP-2 and MMP-9 in CNE1 cells after treatments with the mimic or inhibitor of miR-613 and FN1 as well as the LY294002 treatment examined by Western blot analysis. &P<0.05 compared with the blank group; *P<0.05 compared with the NC mimic group; #P<0.05 compared with the si-NC group; @P<0.05 compared with the miR-613 mimic group; the measurement data were expressed as the mean ± standard deviation; data among multiple groups were compared by one-way ANOVA; the experiment was repeated three times.