FIG 5.
tRNASer isoacceptor transcription levels are decreased in stationary phase relative to exponential phase. tRNASer isoaccepter gene expression was quantified by qRT-PCR on cells grown to exponential and stationary phase in MSgg medium. There was a significant reduction in expression levels all five tRNASer isoacceptor genes in stationary phase relative to exponential phase. The three that recognize UCN codons (trnB-ser1, trnSL-ser1, and trnD-ser) were found to have expression levels lower than those that recognize AGY codons. All serine codons recognized by each of the five tRNASer isoacceptors are listed. Each individual symbol represents an individual data point. Error bars represent the 95% confidence interval.