TABLE 2.
Plasmids used in this study
| Plasmid | Detailsa | Reference or source |
|---|---|---|
| pDG268 | An amyE integration vector with a promoterless lacZ, Ampr Cmr | BGSC |
| pDG1728 | An amyE integration vector with a promoterless lacZ, Ampr Specr | BGSC |
| pDR111 | An amyE integration vector that contains Physpank, Ampr Specr | BGSC |
| pYC121 | An amyE integration vector with a promoterless gfp-mut2, Ampr Specr | 38 |
| pJG01 | amyE::Physpank-serA in pDR111, Ampr Specr | This study |
| pJG02 | amyE::PserA-lacZ in pDG268, Ampr Cmr | This study |
| pJG03 | amyE::Physpank-trnB-ser1 in pDR111, Ampr Specr | This study |
| pJG04 | amyE::Physpank-trnB-ser2 in pDR111, Ampr Specr | This study |
| pJG05 | amyE::Physpank-trnS-ser in pDR111, Ampr Specr | This study |
| pJG06 | amyE::Physpank-trnSL-ser1 in pDR111, Ampr Specr | This study |
| pJG07 | amyE::Physpank-trnD-ser in pDR111, Ampr Specr | This study |
| pJG08 | amyE::PserA-gfp Ampr Cmr | This study |
| pABR174 | amyE::PsdaAB-lacZ in pDG1728, Ampr Specr | This study |
a
Ampr, ampicillin resistance; Cmr, chloramphenicol resistance; Specr, spectinomycin resistance.