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. 2019 Jul 10;201(15):e00188-19. doi: 10.1128/JB.00188-19

FIG 6.

FIG 6

The ClpCP complex is required for optimal growth in a medium that only supports respiratory growth. (A) S. aureus can utilize glutamate as a source of carbon aerobically but not fermentatively. Final growth yields are displayed for the WT (JMB 1100) cultured in the presence or absence of oxygen (fermentative growth) and in defined minimal medium containing the canonical 20 amino acids and glucose (DFM-glucose) or glutamate (DFM-glutamate) as a carbon source or in the absence of a carbon source. (B) A heme auxotroph is unable to utilize glutamate as a carbon source during aerobic growth. Growth profiles are displayed for a hemB::Tn (JMB 6037) strain cultured aerobically in either DFM-glucose or DFM-glutamate. (C and D) A strain lacking ClpC or ClpCP is substantially attenuated for aerobic growth upon DFM-glutamate. Growth profiles are displayed for the WT, ΔclpC (JMB 8025), clpP::Tn (JMB 4898), and ΔclpC clpP::Tn (JMB 8029) strains cultured aerobically in either DFM-glucose (C) or DFM-glutamate (D). Data in all panels represent the averages from duplicate cultures and error bars represent standard deviations. Error bars are displayed for all points but may be too small to see on occasion.