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Purified CobW proteins from C. metallidurans. Lanes 1, 3, and 5 show the His-tagged forms of CobW1, CobW2, and CobW3, respectively; lanes 2, 4, and 5 show the corresponding untagged forms. Lane M, size marker. An SDS-polyacrylamide gel, Coomassie blue stain, and 10 μg proteins were used, except for untagged CobW1 (5 μg). The CobW2 and CobW3 preparations contain a few remaining contaminations.
