FIG 7.

Zincon competition experiment. A 10 μM concentration of CobW1 (A) (squares), CobW2 (B) (triangles), or CobW3 (C) (inverted triangles) was mixed with 10 μM Zincon and various concentrations of zinc chloride. The percentage of zinc saturation was calculated from the Zincon absorption at 480 nm (without zinc) and 620 nm (bound zinc). Controls were 10 μM Zincon without protein (open circles) and 10 μM Zincon plus 10 μM EDTA (filled circles). These controls are given in all panels. Results are from triplicates, and standard deviations are shown.