FIG 5.
Thermodynamics of SBP Cphy2466 binding to sugar ligands measured by isothermal titration calorimetry. Raw binding heats of 15 μM Cphy2466 with 18- by 0.5-μl injections of either 5 mM glucose (A), 0.5 mM cellobiose (B), or 0.5 mM cellohexaose (C). Ordinate axis shows differential power (DP) between the reference and sample cells needed to maintain a zero temperature difference. (D) Integrated binding heats of Cphy2466 binding cellobiose minus the dilution control heats fitted to a single-site binding model to calculate binding enthalpy (ΔH), association binding constant (KA), and the number of binding sites per monomer (n).