FIG 1.

Characterization of the Wzz proteins of P. aeruginosa serotype O13. (A) Gene deletions of wzz₁ and wzz₂ affect OSA chain length. The results indicate that Wzz₁ regulates the long-chain OSA, while Wzz₂ regulates the very-long-chain OSA in serotype O13. (B) Reciprocal swaps of the periplasmic and TM/cytosolic domains of Wzz₁ and Wzz₂. Lane 1, Wzz₁; lane 2, Z2-Z1(prp)-Z2 (Wzz₂ TM/cytosolic domains with Wzz₁ periplasmic domain); lane 3, Wzz₂; lane 4, Z1-Z2(prp)-Z1 (Wzz₁ TM/cytosolic domains with Wzz₂ periplasmic domain). (C) The constructs were expressed in the O13 Δwzz₁ Δwzz₂ double-mutant background. The results indicate that the periplasmic domain is the main determinant of chain length, while the TM/cytosolic regions play a minor role.