FIG 5.

Replacement of conserved residues in ins_α6/7 affects chain length regulation in Wzz₂. (A) Effect of replacing conserved amino acids with alanine. All variants were expressed in the O13 ΔWzz₂ background. The R318A and R333A variants alter the Wzz₂-mediated banding pattern, while the Y203A, E216A, N332A, R341A, and E344A mutations abolish chain length control. The D312A and F314A variants produce increased amounts of very short OSA and some very long OSA. (B) Effect of substituting functionally important residues with like amino acids in Wzz₂. The Y203F, R341K, and E344D variants produce very short OSA, while the D312E, F314Y, N332Q, and R333K variants simultaneously mediated the production of both very short (VS) and very long OSA.