FIG 6.

Stability of Wzz₂ ins_α6/7 mutants determined by whole-cell protein expression and in vivo cross-linking. (Bottom) Whole-cell lysates of O13Δwzz₂ expressing FLAG-tagged variants of Wzz₂ were subjected to SDS-PAGE and immunoblotting with anti-FLAG antibody and detected using chemiluminescence. Several variants express at levels lower than for the wild-type control. (Top) Corresponding whole-cell cross-linked samples of FLAG-tagged Wzz₂ variants. Bands were detected that approximately correspond to the monomeric (M), trimeric (T), and penta-/hexameric (P/H) forms of FLAG-Wzz₂. Numbers on the left are molecular masses, in kilodaltons.