TABLE 1.
Summary of LPS profiles conferred by Wzz2 insα6/7 point mutantsa
| Construct | Location of mutation | Production of: |
||
|---|---|---|---|---|
| Very short OSA | Very long OSA | Protein | ||
| Wzz2 | NA | − | + | + |
| Y203A variant | insα6 | − | − | − |
| E216A variant | insα6 | − | − | − |
| D312A variant | insα7 | + | +/− | +/− |
| F314A variant | insα7 | + | +/− | +/− |
| R318A variant | insα7 | − | + | + |
| N332A variant | insα7 | − | − | − |
| R333A variant | insα7 | − | + | + |
| R341A variant | insα7 | − | − | +/− |
| E344A variant | insα7 | − | − | − |
| D313A variant | insα7 | − | + | NA |
| Y203F variant | insα6 | + | − | NA |
| E216D variant | insα6 | − | − | NA |
| D312E variant | insα7 | + | + | NA |
| F314Y variant | insα7 | + | + | NA |
| R318K variant | insα7 | − | + | NA |
| N332Q variant | insα7 | + | +/− | NA |
| R333K variant | insα7 | + | +/− | NA |
| R341K variant | insα7 | + | − | NA |
| E344D variant | insα7 | + | − | NA |
a
+, appreciable production; +/− intermediate production; −, no appreciable production; NA, not applicable.