Figure 2.

Dehydrocorydaline inhibits cell growth and proliferation in human melanoma cells. (A) Cell morphology of A375 and MV3 melanoma cells after treating with DMSO or the indicated concentrations of DHC for 48 h. Scale bar, 100 μm. (B and C) The effect of DHC on the proliferation rates of A375 and MV3 cells determined by cell counting in the microscope. (D) The effect of different concentrations of DHC treatment for 48 h on the proliferation rate of PIG1 cells determined by MTT assay. (E and F) The effect of DHC on the viability of A375 and MV3 cells. (G) Images and quantifications of A375 and MV3 cells positive for BrdU staining after treating with DMSO or 40 μM DHC for 24 h. Scale bar, 100 μm. All data are shown as the mean ± SD. A two-tailed unpaired Student’s t-test was carried out. *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001.

Abbreviations: DHC, dehydrocorydaline; DMSO, dimethyl sulfoxide; MTT, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide.