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. 2019 Jul 3;10:737. doi: 10.3389/fphar.2019.00737

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Copyright © 2019 Ma, Liu, Liu, Wang, Chang, Li, Li, Hou and Bai

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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ITRAQ-based quantitation phosphoproteomics analysis of lung tissue. (A) Identification of phosphoproteome and displayed the information of phosphorylation of the protein, peptide, and amino acid. (B) Quantitation of phosphoproteome in volcano plots, satisfy multiple differences log2 ratio ≤ 1.3 or ≥0.667 and P-value ≤0.05; red dots: upregulated; green dots: downregulated. (C) The analysis of signaling pathways and function of significant phosphorylated peptides. (D) The heat map analysis of proteins related with inflammation, cancer, and the cytoskeleton, as well as information of identified phosphorylated peptide.