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. 2019 Jul 10;5(2):vez017. doi: 10.1093/ve/vez017

Figure 2.

Figure 2.

The captured viral nucleoprotein, odin, is encoded in tandem copies in the genome of Chaoborus americanus. (A) The odin-encoding contig aligned to the NUKV S genomic segment. Homologous regions are limited to within the nucleoprotein coding region, shown as a solid black bar in the NUKV S segment. In odin, all sequences are derived from NUKV but only region 2–4 is one contiguous copy of the nucleoprotein gene. At position 3, perfect nucleotide identity with position 1 begins. Possible explanations for odin’s contig structure are (1) assembly error, (2) tandem copies, and (3) circular structure. (B) PCR primers directed inward and outward were used to confirm the accuracy of the repeat structure in our assembly. (C) Exonuclease V does not digest nicked or supercoiled circular DNA but does digest linear single- and double-stranded DNA; incubation with exonuclease five prior to PCR removed templates for odin and a nuclear gene, rpl36, but not a mitochondrial target, COI.