Fig. 5.

Myostatin regulates GH and PRL release from GH3 cells. GH3 cells were cultured with 2 and 20 nM myostatin for 48 h, and cell number was assessed at the indicated times (A). Concentrations of GH and PRL were also measured in conditioned medium of GH3 cells cultured for 4 days with myostatin, changing medium with fresh myostatin on day 2 (n = 12/day/dose, P < 0.05, two-way ANOVA; OD490, optical density units at 490 nm) (B and C). GH mRNA was quantified at the indicated days of treatment by qRT-PCR (D). *Significant differences from controls (P ≤ 0.05; n = 12/group). GH, growth hormone; PRL, prolactin; WT, wild-type.