Table 1.
Primers used in qRT-PCR assays
| Primer Gene Symbol | Source | Assay ID | Splice Variants Targeted | Size, bp |
|---|---|---|---|---|
| ACTB | Bio-Rad | qHsaCED0036269 | All | 62 |
| Gapdh | IDT | Rn.PT.39a.11180736.g | All | 85 |
| Gh1 | IDT | Rn.PT.58.44236580.gs | All | 121 |
| HPRT1 | IDT | Hs.PT.58.20881146 | All | 111 |
| IGF-1 | Bio-Rad | qHsaCED0038638 | All | 118 |
| IGFALS | IDT | Hs.PT.58.267 | Exon 1–3b | 103 |
| IGFBP1 | IDT | Hs.PT.58.3620731 | All | 103 |
| Prl | IDT | Rn.PT.58.9831337 | All | 132 |
ACTB, actin beta; bp, base pair; Gapdh, glyceraldehyde-3-phosphate dehydrogenase; Gh1, growth hormone; HPRT1, hypoxanthine phosphoribosyltransferase 1; IDT, Integrative DNA Technologies; IGF-1, insulin-like growth factor-1; IGFALS, IGF acid labile subunit; IGFBP1, IGF-binding protein-1; Prl, prolactin. qRT-PCR, quantitative reverse-transcriptase polymerase chain reaction.