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. 2019 Mar 20;316(6):C792–C804. doi: 10.1152/ajpcell.00010.2019

Fig. 4.

Fig. 4.

Time dependence of currents in the presence of 500 µM external thiocyanate (SCN−). A: families of currents recorded in the same mouse retinal pigment epithelial (RPE) cell in the absence (top) and presence (bottom) of 500 µM SCN−. The membrane potential was held at −60 mV between voltage steps in the range of −120 mV to +50 mV. Open block arrows point to the outward current and inward tail current produced by a voltage step to +50 mV and the closed block arrows indicate the inward current and outward tail current produced by a voltage step to −120 mV. B: voltage dependence of instantaneous and steady-state currents elicited from a holding potential of −60 mV in the absence and presence of 500 µM external SCN−. Instantaneous currents were measured 10 ms after the onset of the voltage steps (●), and steady-state currents were measured 10 ms before their termination (■). Symbols represent means and the bidirectional error bars represent SE; n = 7 cells from two C57BL/6J mice. C: kinetics of the time-dependent decay in currents in 500 µM SCN−. Left: time course of current decay during voltage steps from a holding potential of −60 to 0 mV (top), fitted by a double-exponential function (red trace) or to −120 mV (bottom), and fitted by a single exponential function (red trace). Right: voltage dependence of the time constant (τ; top) and amplitude (A; bottom) of the fast and slow components of the transient current obtained from single or double-exponential fits. Data shown represent means ± SE; n = 7 cells from 2 C57BL/6J mice). D: kinetics of the time-dependent decay in tail currents in 500 µM SCN−. Left: time course of tail current decay at −60 mV following the offset of voltage steps to −120 mV (top) fitted by a single-exponential function (red trace) or to 0 mV (bottom), fitted by a double-exponential function (red trace). Right: voltage dependence of the time constant (top) and amplitude (bottom) of the fast and slow components of the tail current obtained from single or double-exponential fits. Data shown represent means ± SE; n = 5–7 cells from 2 C57BL/6J mice.