Fig. 4.
Cytochrome P450 1B1-deficient (Cyp1b1−/−) retinal astrocytes (ACs) are more adherent. The adhesion properties of retinal ACs were determined by plating them on various extracellular matrix proteins. A: adhesion of Cyp1b1+/+ and Cyp1b1−/− ACs to collagen IV, collagen I, fibronectin, and vitronectin was determined as described in materials and methods. Please note an increase in adhesion of Cyp1b1−/− ACs on fibronectin and vitronectin (*P < 0.05; n = 3). B: localization of F-actin and vinculin in Cyp1b1+/+ and Cyp1b1−/− retinal ACs determined by immunofluorescence staining. Please note increased levels of actin stress fibers and peripheral focal adhesions in Cyp1b1−/− ACs (white arrows). Higher magnifications are shown in panels at bottom. Scale bars = 200 µm (panels at top) and 100 µm (panels at bottom). OD405nm, optical density at 405 nm.