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. 2019 Mar 20;316(6):C767–C781. doi: 10.1152/ajpcell.00021.2019

Fig. 6.

Fig. 6.

Altered production of extracellular matrix proteins in retinal astrocytes (ACs). The conditioned medium (CM) and cell lysates (CL) were collected for analysis of extracellular matrix proteins by Western blot as described in materials and methods. The expression of fibronectin, tenascin C, thrombospondin-1 (TSP1), TSP2, secreted protein acidic and rich in cysteine (SPARC), periostin, and osteopontin was determined using specific antibodies. β‐Actin was used as a loading control for cell lysates. Please note a decrease in the level of TSP1 in cell lysates and an increase in the levels of fibronectin in cell lysates and conditioned medium prepared from cytochrome P450 1B1-deficient (Cyp1b1−/−) ACs compared with Cyp1b1+/+ cells. No TSP1 was detected in the conditioned medium collected from these cells. The expressions of periostin, osteopontin, SPARC, tenascin C, and TSP2 were not detected (**P < 0.01; n = 3).