Fig. 1.

Time- and dose-dependent effects of Thiamet-G (ThG) protein O-linked β-N-acetylglucosamine (O-GlcNAc)ylation in AC16 cells. AC16 cardiomyocytes below passage 13 were used for experiments. A: immunoblot analysis of protein O-GlcNAcylation in AC16 cells exposed to ThG (1 and 5 µM) over 0–6 h. B: quantification of whole lane densitometry representative of O-GlcNAc relative to β-actin from A. C: immunoblot analysis of O-GlcNAcase (OGA) and O-GlcNAc transferase (OGT) in AC16 cells exposed to 1 and 5 µM ThG for 1- and 6-h time points. D: densitometric analysis of immunoblots in C OGA/OGT relative to β-actin. Data are means ± SE; n = 3 replicates per sample. *P < 0.05, different from untreated control. #P < 0.05, different from same dose at different time point.