Fig. 2.

Effect of low-dose Thiamet-G (ThG) treatment (1 µM) on mitochondrial function over time. Oxygen consumption rate (OCR) was measured following 1 µM ThG treatment over increasing time periods. A mitochondrial stress test was performed by establishing basal OCR followed by sequential injections of 1 µg/ml oligomycin, 1 µM FCCP, and 10 µM antimycin A. A: representative OCR traces of mitochondrial stress test. B and C: indexes of mitochondrial function: ATP-linked (B) and nonmitochondrial (C) OCR were calculated. Data are means ± SE; n = 3–5 replicates per sample. *P < 0.05, different from untreated control.