Fig. 2.
Effect of exposure of mice to flagellin on carrier-mediated [3H]thiamin uptake (A) and on levels of expression of mouse thiamin transporters 1 and 2 (mTHTR-1 and mTHTR-2) proteins (B and C) and mRNAs (D and E). Primary pancreatic acinar cells (PACs) were isolated from mice injected with flagellin (5 µg/mouse) for 48 h, and carrier-mediated thiamin uptake was determined as described in materials and methods. Western blot analysis was done using PAC whole cell proteins (40 µg). Levels of protein expression were determined by Western blotting; that of mRNA was determined by qPCR. Data are means ± SE of 3 pairs of mice. **P < 0.01, *P < 0.05.