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Toll-like receptor 5 (TLR-5) mediates the inhibitory effect of flagellin on thiamin uptake by pancreatic acinar cells. Acinar 266-6 cells were transiently transfected with TLR-5 small interfering (si)RNA followed by treatment with flagellin (100 ng/ml for 24 h); thiamin uptake was then determined (see materials and methods). Data are means ± SE of 3 independent experiments. **P < 0.01; NS, not significant.
