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. 2019 Apr 10;316(6):F1162–F1172. doi: 10.1152/ajprenal.00422.2018

Fig. 1.

Fig. 1.

Dichloroacetate (DCA) ameliorates the induction of multiple fibrotic markers in unilateral ureteral obstruction (UUO)-injured kidneys. C57BL/6 male mice were treated with PBS or DCA and subjected to UUO for 7 days. Kidneys were collected to extract whole kidney lysate and examined by immunoblot analysis. protein expression levels from immunoblots were quantified by densitometry analysis and normalized with cyclophilin B expression. Data are expressed as means ± SD; n is the repetition number of parallel experiments. A: representative immunoblot images showing protein level changes of collagen type IV, fibronectin, collagen type I, vimentin, and α-smooth muscle actin (α-SMA). The matched cyclophilin B immunoblots indicate the protein loading. con, Contralateral kidneys; UUO7D, kidneys with 7-day UUO injury. B: densitometry analysis of the collagen type IV level (n = 5, *P = 0.016). C: densitometry analysis of the fibronectin level (n = 4, *P = 0.048). D: densitometry analysis of the collagen type I level (n = 5, *P = 0.041). E: densitometry analysis of the α-SMA level (n = 5, *P = 0.049). F: densitometry analysis of the vimentin level (n = 4).