Skip to main content
. 2019 Apr 10;316(6):F1162–F1172. doi: 10.1152/ajprenal.00422.2018

Fig. 2.

Fig. 2.

Shikonin ameliorates the induction of multiple fibrotic markers in unilateral ureteral obstruction (UUO)-injured kidney. C57BL/6 male mice were treated with vehicle solution (VEH) or shikonin (SK) and subjected to UUO for 7 days. Kidneys were collected to extract whole kidney lysate and examined by immunoblot analysis. Protein expression levels from immunoblots were quantified by densitometry analysis and normalized with cyclophilin B expression. Data are expressed as means ± SD; n is the repetition number of parallel experiments. A: representative immunoblot images showing protein level changes of collagen type I, fibronectin, α-smooth muscle actin (α-SMA), vimentin, and collagen type IV. The matched cyclophilin B immunoblots indicate protein loading. con, Contralateral kidneys; UUO7D, kidneys with 7-day UUO injury. B: densitometry analysis of the collagen type I level (n = 5, *P = 0.005). C: densitometry analysis of the fibronectin level (n = 5, *P = 0.040). D: densitometry analysis of the collagen type IV level (n = 5). E: densitometry analysis of the vimentin level (n = 5). F: densitometry analysis of the α-SMA level (n = 5).