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. 2019 Apr 10;316(6):F1162–F1172. doi: 10.1152/ajprenal.00422.2018

Fig. 3.

Fig. 3.

Dichloroacetate (DCA) reduces interstitial collagen deposition in unilateral ureteral obstruction (UUO)-injured kidneys. C57BL/6 male mice were treated with PBS versus DCA or vehicle solution versus shikonin and subjected to UUO for 7 days. Kidneys were fixed for paraffin-embedded sections. Kidneys were stained by Masson’s trichrome staining to detect collagen deposition. The percent area with collagen deposition in the whole kidney was analyzed by ImageJ. Data are expressed as means ± SD; n is the repetition number of parallel experiments. A: representative images of PBS- or DCA-treated kidneys by Masson’s trichrome staining. CON, contralateral kidneys; UUO7D, kidneys with 7-day UUO injury. Scale bar = 0.2 mm. B: collagen deposition area percentage in PBS- or DCA-treated kidneys after 7-day UUO injury (n = 4, *P = 0.026). C: representative images of vehicle solution- or shikonin-treated kidneys by Masson’s trichrome staining. Scale bar = 0.2 mm. D: collagen deposition area percentage in vehicle solution- or shikonin-treated kidneys after 7-day UUO injury (n = 4).