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. 2019 Apr 3;316(6):F1180–F1190. doi: 10.1152/ajprenal.00089.2019

Fig. 3.

Fig. 3.

NF-κB essential modulator (NEMO) neutralization attenuates peptidyl arginine deiminase-4 (PAD4)-mediated proinflammatory gene expression in HK-2 cells. Recombinant human PAD4 (10 μg/ml for 6 h) treatment significantly induced monocyte chemoattractant protein-1 (MCP-1; top) and macrophage inflammatory protein-2 (MIP-2; bottom) mRNA in HK-2 cells (measured by quantitative RT-PCR). NEMO-binding peptide (NBP; 10 μM for 30 min) pretreatment attenuated the PAD4-mediated induction of these two chemokines in HK-2 cells. *P < 0.05 vs. vehicle; #P < 0.05 vs. PAD4 (n = 5–6 experiments). Error bars represent 1 SE.