Table 1.

Primers used in quantitative RT-PCR to amplify cDNAs based on published GenBank sequences for mouse and human cytokines and annealing temperatures

Primers	Sequence	Annealing Temperature, °C
Mouse MCP-1		60
Sense	5′-ACCTGCTGCTACTCATTCAC-3′
Antisense	5′-TTGAGGTGGTTGTGGAAAAG-3′
Mouse MIP-2		60
Sense	5′-CCAAGGGTTGACTTCAAGAAC-3′
Antisense	5′-AGCGAGGCACATCAGGTACG-3′
Mouse KC		60
Sense	5′-CAATGAGCTGCGCTGTCAGTG-3′
Antisense	5′-CTTGGGGACACCTTTTAGCATC-3′
Human MCP-1		64
Sense	5′-AGCAAGTGTCCCAAAGAAGC-3′
Antisense	5′-CTCAAAACATCCCAGGGGTA-3′
Human MIP-2		64
Sense	5′-CTTGCCAGCTCTCCTCCTC-3′
Antisense	5′-GCTTTCTGCCCATTCTTGAG-3′
Human/mouse GAPDH		65
Sense	5′-ACCACAGTCCATGCCATCAC-3′
Antisense	5′-CACCACCCTGTTGCTGTAGCC-3′

KC, keratinocyte-derived cytokine; MCP-1, monocyte chemoattractant protein-1; MIP-2, macrophage inflammatory protein-2.