Fig. 1.

Absence of Na⁺-glucose cotransporter SGLT1 did not affect the early glomerular and tubular impairment in response to ischemia-reperfusion (IR) but did improve recovery. A: renal mRNA expression of Slc5a1 (Sglt1) and Slc5a2 (Sglt2) were measured on day 16 after IR or sham surgery [normalized to the housekeeping gene hypoxanthine-guanine phosphoribosyltransferase (Hprt)]. B: plasma creatinine rose to similar levels in Sglt1^−/− and wild-type (WT) mice on day 1 after IR, indicating a similar initial glomerular filtration rate (GFR) reduction. On day 16 after IR, plasma creatinine was more fully recovered and lower in Sglt1^−/− versus WT mice. C: direct measurement on day 14 after IR confirmed higher GFR values in Sglt1^−/− versus WT mice. D: urinary levels of kidney injury molecule (KIM)-1 were similarly increased in Sglt1^−/− and WT mice on day 1 after IR, suggesting similar initial proximal tubule injury. E and F: transient increases in urinary glucose-to-creatinine ratios (E) and in the fractional excretion of glucose (FE-glucose; F) in both genotypes on day 1 after IR, with higher levels observed in Sglt1^−/− versus WT mice, indicating a robust contribution of SGLT1 to glucose reabsorption after IR. G: blood glucose was significantly reduced in Sglt1^−/− mice on day 1 after IR versus sham surgery. H: a transient decrease in body weight was observed in Sglt1^−/− mice after IR. Results are means ± SE; n = 7–8 mice/group in A, n = 6–8 mice per group and per time point in B; n = 4 sham mice/group and 6–7 IR mice/group in C; n = 6–8 mice/group in D, and n = 5–8 mice per group and per time point in E−H. #P < 0.05 vs. sham and *P < 0.05 vs. WT mice using two-way ANOVA and Holm-Sidak post hoc analysis for multiple comparisons.