Fig. 2.

The C-terminus of CXorf67 is crucial for PRC2 binding and inhibition. (A) Staining of HEK293T cells expressing CXorf67 truncates or the full-length protein using an H3K27me3 antibody. Cells expressing CXorf67-C or CXorf67-Full show a reduced H3K27me3 signal. Scale bar: 20 µm. (B) Western blot analysis of H3K27me3 levels in HEK293T cells expressing CXorf67 truncates or the full-length protein. A strong downregulation of H3K27me3 can be observed for CXorf67-C and CXorf67-Full. (C) Heatmap depicting significantly (P < 0.05) deregulated genes (n = 198) upon overexpression of CXorf67-Full in HEK293T cells. A strong deregulation of the same genes can also be observed for CXorf67-C expressing cells (n = 3 per condition). The scale bar depicts the z-scores of the gene expression levels. (D) Comparison of expression levels for FGF13, PLK2, and PLAG1 in transduced cell lines (n = 3), untransduced control cells (n = 3), and PFA tumors (n = 55). (E) GO enrichment analysis of CXorf67-Full upregulated genes (n = 188). X-axis depicts the p-value (log₁₀). (F) GSEA showing significant enrichment of PRC2 target genes in CXorf67-C and CXorf67-Full cells compared with untransduced control cells. No significant enrichment is observed for the other truncated variants. A representative plot for CXorf67-Full is shown. NES: normalized enrichment score.