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Astrocytoma. H&E shows a hypercellular tumor composed of pleomorphic tumor cells (A). This tumor is immunoreactive for IDH1 R132H (B) and TP53 (C), but has lost ATRX expression (D). Fluorescence in situ hybridization (FISH) showed retention of 1p (E) and 19q (F). Scale bar A–D = 100 microns; scale bar E–F = 20 microns. FISH images courtesy of Kathleen A. Kaiser-Rogers, PhD.
