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. 2019 Jul 4;11:172. doi: 10.3389/fnagi.2019.00172

Figure 3.

Figure 3

Perivascular cholinergic contact at the basement membrane. (A–M) Representative images of the 3D reconstruction of the NVU in capillaries (A,B,H,I), arteries (C,D,J,K) and veins (E,F,L,M) in the hippocampus (A–F) and cortex (H–M) of control (A,C,E,H,J,L) and saporin-treated mice (B,D,F,I,K,M). ChAT-positive fibers are shown in red, collagen IV is shown in blue and SMA is shown in green. (G,N) Quantification of the surface area of contact between ChAT-positive fibers and collagen IV demonstrated a significant decrease in contact at the arteries of saporin-treated animals in both the hippocampus (G) and cortex (N). Data represent mean ± SEM. *p < 0.05, two-way ANOVA with Sidak’s post hoc test. Scale bars = 20 μm.