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. 2019 Jul 4;11:172. doi: 10.3389/fnagi.2019.00172

Figure 5.

Figure 5

Perivascular cholinergic contact at perivascular astrocytes. (A–M) Representative images of the 3D reconstruction of the NVU in capillaries (A,B,H,I), arteries (C,D,J,K) and veins (E,F,L,M) in the hippocampus (A–F) and cortex (H–M) of control (A,C,E,H,J,L) and saporin-treated mice (B,D,F,I,K,M). ChAT-positive fibers are shown in red, collagen IV is shown in blue, SMA is shown in green and glial fibrillary protein (GFAP) is shown in turquoise. (G,N) Quantification of the surface area of contact between ChAT-positive fibers and astrocyte endfeet found no differences between control and saporin-treated animals in any vessels of the hippocampus (G), but a significant decrease in contact at the arteries of saporin-treated animals the cortex (N). Data represent mean ± SEM. **p < 0.01, two-way ANOVA with Sidak’s post hoc test. Scale bars = 20 μm.