Fig. 4.

Function and pathway enrichment of DEGs in the groups treated with DMSO, CLA (10 μM), or NO₂-CLA (10 μM). A: top GO terms and KEGG pathways in the overlapping DEGs identified from the comparisons of NO₂-CLA, DMSO, and CLA. B: network of the top GO terms and pathways. C: heat map of the DEGs in two pathways essential to endothelial cell (EC) function. D: qPCR validation of the novel NO₂-CLA responsive genes in the pathways in C. data are presented as means ± SE, n = 4 per group. *P < 0.05; **P < 0.01 by one-way ANOVA followed by Dunnett test, compared with the DMSO group.