Table 1.
Primers used for real-time PCR
| Gene | Primer Sequence (5′-3′); Forward/Reverse | Accession No. |
|---|---|---|
| β-Actin | GTCCACCGCAAATGCTTCTAA/TGCGCATTTATGGGTTTTGTT | NM_205518.1 |
| ACADL | GACATCGGCACTCGGAAGA/CCTGGTGCTCTCCCTGAAGA | NM_001006511.2 |
| AGPAT2 | GCCAAACACCGAAGGAACAT/CCATGGCATCCCCAGAGTT | XM_015279793.1 |
| ATGL | GCCTCTGCGTAGGCCATGT/GCAGCCGGCGAAGGA | NM_001113291.1 |
| C/EBPα | CGCGGCAAATCCAAAAAG/GGCGCACGCGGTACTC | NM_001031459.1 |
| C/EBPβ | GCCGCCCGCCTTTAAA/CCAAACAGTCCGCCTCGTAA | NM_205253.2 |
| DGAT2 | TTGGCTTTGCTCCATGCAT/CCCACGTGTTCGAGGAGAA | XM_419374.5 |
| FABP4 | CAGAAGTGGGATGGCAAAGAG/CCAGCAGGTTCCCATCCA | NM_204290.1 |
| LPL | GACAGCTTGGCACAGTGCAA/CACCCATGGATCACCACAAA | NM_205282.1 |
| NPY | CATGCAGGGCACCATGAG/CAGCGACAAGGCGAAAGTC | NM_205473.1 |
| NPYR2 | TGCCTACACCCGCATATGG/GTTCCCTGCCCCAGGACTA | NM_001031128.1 |
| PPARγ | CACTGCAGGAACAGAACAAAGAA/TCCACAGAGCGAAACTGACATC | NM_001001460.1 |
| SREBP1 | CATCCATCAACGACAAGATCGT/CTCAGGATCGCCGACTTGTT | NM_204126.1 |
ACADL, acyl-CoA dehydrogenase, long chain; AGPAT2, 1-acylglycerol-3-phosphate O-acyltransferase 2; ATGL, adipose triglyceride lipase; C/EBPα, CCAAT/enhancer-binding protein-α; C/EBPβ, CCAAT/enhancer-binding protein-β; DGAT2, diacylglycerol O-acyltransferase 2; FABP4, fatty acid binding protein 4; LPL, lipoprotein lipase; NPY, neuropeptide Y; NPYR2, NPY receptor 2; PPARγ, peroxisome proliferator-activated receptor-γ; SREBP1, sterol regulatory element-binding transcription factor 1.