Fig. 4.

Infiltrated T cells have innate-like inflammatory potential. A: hyperlipidemic CD45.2 wild-type (WT) mice received adoptive transfer of CD45.1 Ova-specific splenocytes 2 wk after partial carotid artery ligation (PCAL) and were immunized with SIINFEKL and agonist anti-CD137 mAb. Vessels were harvested 4 days postimmunization (dpi). B: 4 dpi with SIINFEKL and agonist anti-CD137 mAb, splenocytes have a robust innate-like response to the cytokine combination of IL-2 + IL-36. Cells were stimulated in vitro, and secreted IFNγ was measured by ELISA analysis of supernatant. ^nsP > 0.1, **P < 0.03 by series of paired t-tests; n = 5–8/group. C: this innate-like secretion was also seen in cells isolated from the abdominal aorta, a physiological site of low and disturbed flow (LDF) at intercostal branch points, but not in cells isolated from the linear carotid, a vessel subjected to laminar high flow (right). ^nsP = 0.2, *P = 0.015 by paired t-test; n = 2–8 mice/group. D: cells isolated from PCAL-ligated carotids have innate-like responses to cytokines (IL-2 + IL-36) and also secrete IFNγ in response to phorbol 12-myristate 13-acetate + ionomycin (PMAi), as measured by ELISA. *P < 0.02 by paired t-tests; n = 6 mice/group. ▲ and ◆, Data points from individual mice.