Fig. 6.

A representative experiment using the autonomous autopatcher for consecutive patch-clamp recordings in layer 5 of mouse visual cortex in vivo. From 40 loaded pipettes, 34 attempts to record are made in two craniotomies, resulting in four whole cell recordings for visual cortex functional characterization. Operator denotes manual intervention (e.g., switching craniotomy, tending to animal welfare, optimizing anesthesia conditions). Recordings 3 and 4 were automatically terminated early after they fell below quality thresholds. A: current clamp recordings of the four cells recording during this single experiment. B: current injection amplitudes and durations corresponding to the recordings in A. C: timeline of events and duration of the activities during this 4-h experiment.